两种悬浮填料在ASBBR厌氧氨氧化系统中的性能比较与微生物解析Performance comparison and microbial analysis of two kinds of suspend
发布日期：2020-04-17 浏览次数：
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核心提示：两种悬浮填料在ASBBR厌氧氨氧化系统中的性能比较与微生物解析Performance comparison and microbial analysis of two kinds of suspended fillers in the anammox system of ASBBRWang Jun1, Yu Deshuang1, Wang Xiaoxia1, Song Xinxin2, Li Chuanju2, Li Jia2, Lin Jia2,*, Liu Jie2,*关键词：城市生活污水 厌氧
- 两种悬浮填料在ASBBR厌氧氨氧化系统中的性能比较与微生物解析
- Performance comparison and microbial analysis of two kinds of suspended fillers in the anammox system of ASBBRWang Jun1, Yu Deshuang1, Wang Xiaoxia1, Song Xinxin2, Li Chuanju2, Li Jia2, Lin Jia2,*, Liu Jie2,*
- 基金项目：国家水体污染控制与治理科技重大专项(2017ZX07102-003); 国家自然科学基金(No. 51978348, 51778304)
- 摘要：选取T1聚乙烯和T2聚氨酯两种悬浮填料投入两套相同厌氧序批式生物膜反应器(ASBBR) R1和R2中 ,以城市生活污水为配水基质 ,考察两种填料在ASBBR的性能及菌群结构特征 ,选出更适宜厌氧氨氧化菌(ANAMMOX)生长的填料.结果表明 :R1和R2反应器分别在第93 d 和73 d 成功启动厌氧氨氧化 ,并实现稳定运行.稳定运行阶段R1、R2系统总无机氮(TIN)平均去除率分别达88.34%、87.97%.此外 ,运行第150 d时 ,测得单个T1、T2填料的ANAMMOX活性分别为5.70 mg·g-1·h-1和3.70 mg·g-1·h-1.同时 ,扫描电子显微镜(SEM)表征结果证明 ,球状菌在T1填料上的数量比同倍数下T2填料上多 ,且杂菌较少. 高通量测序结果显示 ,T1、T2填料上Candidatus anammoxoglobus菌属相对丰度分别为75.29%和38.23%.本研究表明 ,相比于T2聚氨酯填料 ,T1聚乙烯填料更适宜ANAMMOX菌的富集.
- Abstract：In order to select a more suitable kind of suspended carrier for anaerobic ammonium oxidation (ANAMMOX) bacteria, two types of carriers which named T1 polyethylene and T2 polyurethane were tested in two parallel sets of the anaerobic sequencing batch biofilm reactor (ASBBR) R1 and R2. The performance and bacterial structure on two different kinds of carrier in ASBBR were studied with municipal sewage as water distribution matrix. The experiment showed that R1 and R2 reactors successfully started on the 93rd day and the 73rd day respectively and both achieved stable operation. During the stable phase, the average TIN removal efficiency of R1 and R2 had reached up to 88.34% and 87.97%, respectively. In addition, the activity of ANAMMOX on a single T1 and T2 carrier was 5.70 mg·g-1·h-1 and 3.70 mg·g-1·h-1 on the 150th day. Meanwhile, scanning electron microscope(SEM) characterization results indicated that the number of globular bacteria on T1 carrier was more than that on T2 at the same magnification and also has fewer heterozygous bacteria. The high-throughput sequencing result demonstrated that the relative abundance of Candidatus anammoxoglobus on T1 and T2 carrier was 75.29% and 38.23%, respectively. This study concludes that T1 polyethylene carrier is more suitable for the enrichment of ANAMMOX bacteria than T2 polyurethane carrier.
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